Construction of an Amperometric Lactate Biosensor Based on Immobilization of Lactate Dehydrogenase Nanoparticles onto Pencil Graphite Electrode
نویسندگان
چکیده
منابع مشابه
Development of a screen-printed amperometric biosensor for the determination of L-lactate dehydrogenase level.
We attempted to develop a screen-printed biosensor for the amperometric determination of L-lactate dehydrogenase (LDH) level on the basis of NAD(+)/NADH-dependent dehydrogenase reaction. The printing ink for the working electrode consisted of L-lactate, NAD(+), composite polymer of hydroxyethyl cellulose with ethylene glycol, 3,4-dihydroxybenzaldehyde (3,4-DHB) as an electron transferring media...
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The electrochemical entrapment of polyaniline (PANI) onto sol-gel derived tetraethylorthosilicate (TEOS) films deposited onto indium-tin-oxide (ITO) coated glass has been utilized for immobilization of lactate dehydrogenase (LDH). The performance of these sol-gel/PANI/LDH electrodes has been investigated as a function of the lactate concentration, applied potential, pH of the medium and interfe...
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ژورنال
عنوان ژورنال: Biochemistry & Analytical Biochemistry
سال: 2017
ISSN: 2161-1009
DOI: 10.4172/2161-1009.1000331